PERIPHERAL NEUROPATHIES

  • Peripheral neuropathy is the result of damages of the peripheral nervous system. These troubles can be caused either by a trauma or diseases as for example diabetes.
    NEUROFIT proposes animal and cellular models able to evaluate the neuroprotective activity of your coumpounds.


    Models of CHEMOTHERAPY INDUCED NEUROPATHY

    Cisplatine, vincristine and taxol are widely used as antimitotic drugs for treating various cancers. However, their employment is often limited by their neurotoxicity ( Quasthoff et Hartung, 2002 ). Counteracting this side effect without affecting their anti-cancer activities is a promising developmental strategy.


  • Sensory neurons (DRG)

    Purified cultures of sensory neurons are injured by chemotherapeutic drugs (cisplatine 3 µg/ml, vincristine 7.5 ng/ml or taxol 0.5 µg/ml). The aim of this study is to investigate the putative protective effect of tested compounds on this damage. Neuronal survival is assessed by measuring acid phosphatase activity after exposure to chemotherapy and tested compound.
  • CO-CULTURE of SENSORY NEURONS and SCHWANN CELLS

    Co-cultures from sensory neurons and Schwann cells are injured by chemotherapeutic drugs (cisplatine 0.5 µg/ml, vincristine 1 ng/ml or taxol 0.5 µg/ml). The aim of this study is to investigate the putative protective effect of tested compounds on this damage. Global cellular survival is assessed by measuring acid phosphatase activity after exposure to chemotherapy and tested compound.

    ☐ CISPLATIN intoxication


  • Purified cultures of sensory neurons :
    Cisplatin induced cell death in sensory neurons.
    At each dose of cisplatin, NGF at 5 ng/ml is able to protect neurons against the ciplatin damage.

  • Co-cultures of sensory neurons and Schwann cells :
    Cisplatin induces cell death in a dose dependant manner.
    At each dose tested, NGF at 5 ng/ml is able to protect neurons against ciplatin damage.
    ☐ TAXOL intoxication


  • Purified cultures of sensory neurons :
    Taxol induced sensory neurons death.
    NGF is able to protect sensory neurons against this injury.

  • Co-cultures of sensory neurons and Schwann cells :
    Taxol induces sensory neuron cell death.
    NGF is able to protect sensory neurons against this injury.
    ☐ VINCRISTINE intoxication


  • Purified cultures of sensory neurons :
    Vincristine induced sensory neurons death.
    NGF is able to protect sensory neurons against this injury.


  • Co-cultures of sensory neurons and Schwann cells :
    Vincristine induces cell death in sensory neurons in a dose dependant manner.
    At each dose tested, NGF at 5 ng/ml is able to protect neurons against the vincristine damage.

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References :

  • Quatschoff S. and Hartung H.P Chemotheray-induced peripheral neuropathy. J. Neurol. 249:9-17. (2002)

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