PERIPHERAL NEUROPATHIES

  • Peripheral neuropathy is the result of damages of the peripheral nervous system. These disorders can be caused either by a trauma or diseases as for example diabetes.
    NEUROFIT proposes animal and cellular models usefu for the evaluation of the neuroprotective activity of your coumpounds.


    Cellular model: Basal Condition

    Damage sensory neurons are involved in various symptoms of peripheral neuropathy such as pain, numbness, tingling, burning, …
    Neurofit developed 3 different types of primary culture to assess the neurotrophic, neuroprotective or neurotoxic properties of compounds.

  • Sensory neurons and Schwann cells co-culture from rat embryos

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    mimics the in vivo nerve
    axon/neuron-glia interaction
    allows to study the involvement of Schwann cells on the survival of sensory neurons

  • Purified sensory neurons culture from rat embryos

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    focused approach
    to study direct neurons responses to stimuli

  • Culture of neurons from adult rat dorsal root ganglia

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    neurite outgrowth without stimulation (NGF)
    allow the use of naïve or pathological rodents
    reflect the broad diversity of mature neuronal population: mimic the in vivo characteristic
  • Basal condition: Embryonic Sensory neurons and Schwann cells co-culture

  • Compound testing :

    Neurotrophic
    Neuroprotectant
    or neurotoxic

  • Endpoints :

    Neuronal activity or survival (phosphatase acid activity)
    Neuronal death (LDH release)
    Measure of neurite network



    • Phosphatase acid
      NGF increases the basal activity of embryonic sensory neurons and Schwann cells coculture in a dose dependant manner.

    • Neurite network
      NGF increases the neuritogenesis of sensory neurons.

    • Phosphatase acid
      Cisplatin (0.5 µg/mL), Taxol (7.5 ng/mL) and Vincristine (1 ng/mL) induces cell death in embryonic sensory neurons and Schwann cells co-culture.

    • extracellular LDH
      Cisplatin (0.5 µg/mL), Taxol (7.5 ng/mL) and Vincristine (1 ng/mL) induces cell death in embryonic sensory neurons and Schwann cells co-culture.

    • Neurite network
      Taxol (7.5 ng/mL) and Vincristine (1 ng/mL) induce a destruction of neurite network.



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  • Phosphatase acid
    NGF increases the basal activity of embryonic sensory neurons.

  • Phosphatase acid
    Cisplatin(2 µg/mL), Taxol (0.5 µg/mL) and Vincristine (5 ng/mL) induces cell death in embryonic sensory neurons.

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  • Basal condition: Adult dorsal root ganglia culture

  • Primary culture of adult neurons :

    Although adult neurons may behave biologically differently than embryonic neurons, they are rarely used in research because culturing neurons from adult animals is challenging and often returns poor cell yield.
    NEUROFIT can reliably culture adult rat sensory neurons. The assay is cost-effective and is suitable for routing assessment of the neurotrophic, neuroprotective or neurotoxic properties of your test compounds.



  • Mean of neurite length (% of control)
    Cultured adult neurons are pharmacologically responsive to NGF stimulation as shown by the dose-dependent increase in the neurite length.
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  • Cisplatin treatment

    Peripheral neuropathy is the result of damages of the peripheral nervous system induced by insult such as the neurotoxicity of cisplatine.

  • Taxol treatment

    Peripheral neuropathy is the result of damages of the peripheral nervous system induced by insult such as the neurotoxicity of taxol.

    Vincristine treatment

    Neurofit developed this model to assess the neuroprotective properties of compounds aiming to counteract chemotherapy side effects.