Model of β-Amyloid-induced neurotoxicity in rat Hippocampal neurons

Newsletter # 13

Cellular models

Alzheimer disease (AD) is the most common cause of dementia. It is a neurodegenerative disease characterized by an extracellular accumulation of neurotoxic fibrillar amyloid beta peptide (Aβ). Intoxication of neuronal cultures with Aβ has been widely used to understand some of the mechanisms of cell death in AD and thus represents an instrumental in-vitro system to evaluate the efficiency of new drug candidates.

NEUROFIT proposes robust in vitro assay to test to potency of novel compound to protect hippocampal neurons against Aβ induced neurotoxicity.

  • In this assay, 9-day-old hippocampal neuron cultures are injured with the peptide β-amyloid 1-40 (Aβ 1-40). The neuroprotective effect of compounds is evaluated based on their ability to inhibit the damage to the neurons.
    Neuronal survival is assessed by measuring MTT activity and /or neuronal death is assessed by measuring LDH activity in the media.

  • Reduced cell viability (MTT assay) in hippocampal neuron culture in response to increasing concentration of Aβ 1-40

    * : significantly different as compared to control group

  • Increased cell death (LDH assay) in hippocampal neurons in response to increasing doses of Aβ 1-40

    * : significantly different as compared to control group

NEUROFIT offers a range of validated in vitro and in vivo screening tests for psychiatry and neurology.

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