Newsletter # 33
The anxiolytic effect of diazepam (Valium ®) shown in the Light-Dark test in the mouse
Diazepam produces a dose-dependent reduction of time spent in the lit box, suggesting an anxiolytic effect.
The sedative effect of diazepam for doses ≥ 2 mg/kg demonstrated in the Open-field test
Diazepam ≥ 2 mg/kg is associated with reduced locomotion in the open-field which suggests a sedative effect at doses higher than 2 mg/kg.
Reversion of CCK-4 - induced panic anxiety in the Light-Dark test in mice
Injection of CCK-4 (cholecystokinin tetrapeptide) enhances the anxiety status of mice. The panic effect of CCK-4 is suppressed by Diazepam treatment.
Moreover, Neurofit also offers a variant of the Light-Dark test in order to mimic the CCK-4-induced panic anxiety as modelled in human healthy volunteers. Other anxiety tests such as the
Elevated Plus Maze and Marble Burying test
are also available at Neurofit.
CCK-4 - induced panic anxiety has also been validated in the rat Elevated Plus Maze
For a complete listing of tests and models, please refer to our website. If you need further information, please do not hesitate to contact us.
We look forward to hearing from you.