Parkinson disease model: 6-OHDA-mediated neuronal death

Newsletter # 40


Cellular models

Parkinson's disease (PD) is a neurodegenerative disorder with motor symptoms caused by the loss of dopaminergic (DA) neurons in the striatum. 6-hydroxydopamine (6-OHDA) is a neurotoxin widely used in lab animals to produce striatal dopamine depletion.


    COST-EFFECTIVE SCREENING MODEL
    FOR PARKINSON'S DISEASE PROJECT


Microscopic field showing a dopaminergic neuron (green) surround by a population of mesencephalic neurons (blue nuclei). Note that dopaminergic neurons are a subpopulation of mesencephalic neurons.




NEUROFIT uses 6-OHDA in primary culture of mesencephalic neurons to provide cellular model featuring the neuropathology of PD.


  • Example of the effect of neuroprotective treatments against 6-OHDA-mediated death of dopaminergic neurons.


    BDNF and GDNF : neurotrophins
    PD150606 : calpain inhibitor
    z-VAD-fmk : caspase inhibitor
    NAC (N-acetyl-cysteine) : antioxidant
  • Stimulation of neurotrophin pathways


  • Inhibition of Calpain pathways


  • Inhibition of Caspase pathways


  • Inhibition of oxidative stress



NEUROFIT offers a range of validated in vitro and in vivo screening tests for psychiatry and neurology.

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